发表于: 2/03/2005 15:31 发表主题: Mass spectrometry
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Did anybody compare results from different MS facilities? I tries several places and results are not always satisfied.

Can anyone tell me which facility is good at analyze membrane proteins?

Thanks
  • BBSBB
  • 注册于:2005-02-02
  • 帖子:41
发表于: 2/03/2005 15:45 发表主题:
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你要分析什么啊?
发表于: 2/03/2005 16:26 发表主题:
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BBSBB 写到:
你要分析什么啊?


a membrane protein. From SDS-PAGE gel.
发表于: 2/03/2005 16:50 发表主题:
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To identify the protein or anything else?

confused
发表于: 2/03/2005 17:16 发表主题:
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Wild_Goose 写到:
To identify the protein or anything else?

confused


In other word: to find out what the protein is.
  • BBSBB
  • 注册于:2005-02-02
  • 帖子:41
发表于: 2/03/2005 17:39 发表主题:
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纯化,(用酶切)-质谱,查谱!是不是已知蛋白。
用(质谱结果)已知的氨基酸----DNA,PCR.



哈哈,你可能知道得更多。。
colder98 写到:
Wild_Goose 写到:
To identify the protein or anything else?

confused


In other word: to find out what the protein is.

发表于: 2/04/2005 09:18 发表主题:
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BBSBB 写到:
纯化,(用酶切)-质谱,查谱!是不是已知蛋白。
用(质谱结果)已知的氨基酸----DNA,PCR.



哈哈,你可能知道得更多。。
colder98 写到:
Wild_Goose 写到:
To identify the protein or anything else?

confused


In other word: to find out what the protein is.


You got it. But different labs (facilities) get different results. I am looking for some "reliable" facilities.
发表于: 2/04/2005 11:31 发表主题: Mass Spec
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Colder,

Our lab has had consistent good luck with Steve Gygi's facility. He is probably the best one around. On the other hand, I would not recommend the DFCI core facility. And finally if you can wait, Jarod Marto is setting up another facility and he is very good too. Good luck!
发表于: 2/04/2005 13:30 发表主题: Re: Mass Spec
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CrystalClear 写到:
Colder,

Our lab has had consistent good luck with Steve Gygi's facility. He is probably the best one around. On the other hand, I would not recommend the DFCI core facility. And finally if you can wait, Jarod Marto is setting up another facility and he is very good too. Good luck!


I tried twice at Gygi's lab without any luck. I was suspecting that I was dealing with an unkown protein. Maybe I should try it again since it has been a year. The database should have been upgraded.

Thanks a lot.