发表于: 3/03/2005 16:48 发表主题: Does anyone work on Calcium flux?
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I am looking for a ctrl reagent that can be used to trigger intracellular calcium flux (not PMA/Ionomycin).

Can anyone give me an idea?

Thanks,
发表于: 3/04/2005 09:24 发表主题: Calcium flux
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Colder,

I am more familiar with Ionomycin. However, my lab also uses Thapsigargan. It affects intracellular calcium flux through the ER. If you want more info, check my lab's 2003 Science paper from Scorrano or any of Oakes's recent papers.
发表于: 3/04/2005 10:25 发表主题: Re: Calcium flux
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CrystalClear 写到:
Colder,

I am more familiar with Ionomycin. However, my lab also uses Thapsigargan. It affects intracellular calcium flux through the ER. If you want more info, check my lab's 2003 Science paper from Scorrano or any of Oakes's recent papers.


Thank you CrystalClear:

It seems a very good reagent for my experiment. I wonder if I get a little bit from your lab. I have some cells ready today and I want to test it if I can get the reagent.

rose rose rose rose rose rose rose rose rose rose rose rose rose rose rose rose rose rose rose
发表于: 3/04/2005 10:53 发表主题: Thapsigargan
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Colder,

show me the MONEY, and we can talk Laughing

In any case, I just hunted down a small amount of Thapsigargan down for you. Oakes is not in the lab today so I had to scavenge around. Just come over to my lab and pick it up this morning.
发表于: 3/04/2005 10:56 发表主题: Re: Thapsigargan
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CrystalClear 写到:
Colder,

show me the MONEY, and we can talk Laughing

In any case, I just hunted down a small amount of Thapsigargan down for you. Oakes is not in the lab today so I had to scavenge around. Just come over to my lab and pick it up this morning.


If the experiment works, I will treat you lunch/dinner whatsoever.
Please write me your lab number to my mailbox. Thanks a lot. rose rose rose
发表于: 3/04/2005 11:04 发表主题:
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Hmmm...a Nikon D70 will be nice... wink
发表于: 3/04/2005 11:42 发表主题:
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Thank you crystalclear!!
发表于: 3/04/2005 17:16 发表主题:
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CC:

I think the reagent from you is a very good ctrl. One more experiment needs to be done to confirm it.

Thank you so much!! rose
发表于: 3/04/2005 23:38 发表主题:
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Now I am happy!!! Just got the repeat result.
Perfect ctrl. One more experiment next week!! You made my day, CC. Thank you so much!!

happy happy Laughing Laughing
发表于: 3/06/2005 10:12 发表主题: You are welcome
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colder98 写到:
Now I am happy!!! Just got the repeat result.
Perfect ctrl. One more experiment next week!! You made my day, CC. Thank you so much!!


Colder,

You are very welcome! I am glad to be of help.

happy happy happy