• BBSBB
  • 注册于:2005-02-02
  • 帖子:41
发表于: 3/05/2005 13:26 发表主题: About realtime PCR
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Hi guys


that if a nucleotide in primer (~20 nucleotides ) is wrong will result in a wrong result?

thanks
发表于: 3/05/2005 17:01 发表主题: Re: About realtime PCR
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BBSBB 写到:
Hi guys


that if a nucleotide in primer (~20 nucleotides ) is wrong will result in a wrong result?

thanks


Never met the situation before. The safe way is to get a correct primer which won't cost so much. Otherwise, if the mismatch is on primer I guess it should be fine, but if it's on probe, you probably need to be very careful.